<b>Cultivation of immature <i>Capsicum</i> spp. embryos for incompatible-crossing embryo rescue

LLC 18 Embryo Rescue Sandra

Competence of Immature Maize Embryos for Agrobacterium-Mediated Gene Transfer.

Agrobacterium-mediated transfer of viral sequences to plant cells (agroinfection) was applied to study the susceptibility of immature maize embryos to the pathogen. The shoot apical meristem of immature embryos 10 to 20 days after pollination from four different maize genotypes was investigated for competence for agroinfection. There was a direct correlation between different morphological stag...

Genetic transformation using maize immature zygotic embryos.

Epidermal and subepidermal cells in the abaxial, basal region of the maize (Zea mays L.) immature zygotic embryo (IZE) scutellum can be induced by exogenous auxin to proliferate and undergo somatic embryogenesis. Successful genetic transformation of IZEs depends not only on optimizing transformation parameters for these totipotent cells, but also on achieving high embryogenic callus induction f...

Virulence of Francisella spp. in chicken embryos.

We examined the utility of infecting chicken embryos as a means of evaluating the virulence of different Francisella sp. strains and mutants. Infection of 7-day-old chicken embryos with a low dose of F. novicida or F. tularensis subsp. holarctica live vaccine strain (LVS) resulted in sustained growth for 6 days. Different doses of these two organisms were used to inoculate chicken embryos to de...

The effects of BAP, IBA and genotypes on in vitro germination of immature walnut embryos

A prerequisite for most plant transformation systems is an efficient and reliable method to regenerate phenotypically normal plants. The present studies were undertaken with a view to the effects of Benzyl amino purine (BAP) and Indol butyric acid (IBA) hormones and genotypes on in vitro germination of immature embryos in walnut (Juglans regia L.). The immature embryos of walnut were excised fr...